The Mouse PTGS2 (COX-2) Prostaglandin Endoperoxide Synthase 2 ELISA Kit is specifically designed for the accurate measurement of PTGS2 levels in mouse biological samples such as serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PTGS2, also known as cyclooxygenase-2 (COX-2), is an important enzyme involved in the production of prostaglandins, which play a crucial role in inflammation and pain.
Dysregulation of PTGS2 has been implicated in various diseases such as cancer, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Mouse PTGS2 (COX-2) Prostaglandin Endoperoxide Synthase 2 ELISA Kit is an essential tool for researchers looking to investigate the role of PTGS2 in disease pathogenesis and therapeutic targeting.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PTGS2/COX-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PTGS2/COX-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PTGS2/COX-2. You can calculate the concentration of Mouse PTGS2/COX-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
84-98
94-105
Average (%)
101
90
99
1:4
Range (%)
91-105
81-92
85-96
Average (%)
97
87
91
1:8
Range (%)
90-104
87-102
81-93
Average (%)
97
93
88
1:16
Range (%)
88-104
78-92
90-104
Average (%)
95
85
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
90-101
96
Cell culture media (n=5)
95-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
0.9
3.42
0.52
0.82
3.72
Standard deviation
0.03
0.05
0.15
0.03
0.04
0.18
C V (%)
6
5.56
4.39
5.77
4.88
4.84
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PTGS2/COX-2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PTGS2/COX-2 in samples. No significant cross-reactivity or interference between Mouse PTGS2/COX-2 and analogues was observed.
