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Mouse Protein Kinase C Delta (PKCd) ELISA Kit

SKU:
MODL01053
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28867
Sensitivity:
0.54ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
PKC-D,MAY1,PRKCD,SDK1,NPKC-Delta,Tyrosine-protein kinase PRKCD,Sphingosine-dependent protein kinase-1
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Protein Kinase C Delta (PKCd) ELISA Kit

The Mouse Protein Kinase C Delta (PKCD) ELISA Kit is specifically designed for the precise measurement of PKCD levels in mouse serum, plasma, and cell lysates. This advanced kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes.PKCD is a key enzyme belonging to the protein kinase C family, playing a critical role in regulating various cellular processes, including cell growth, differentiation, and apoptosis. Dysregulation of PKCD has been linked to numerous diseases, making it a valuable biomarker for studying pathophysiological mechanisms and developing targeted therapies.

With the Mouse Protein Kinase C Delta (PKCD) ELISA Kit, researchers can confidently investigate the functional role of PKCD in disease progression and explore its potential as a therapeutic target. Elevate your research with this cutting-edge kit and uncover new insights into cellular signaling pathways and disease mechanisms.

Product Name:Mouse Protein Kinase C Delta (PKCd) ELISA Kit
Product Code:MODL01053
Size:96 Assays
Target:PKCd
Synonyms:PKC-D, MAY1, PRKCD, SDK1, NPKC-Delta, Tyrosine-protein kinase PRKCD, Sphingosine-dependent protein kinase-1
Detection Method:Competition
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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