Mouse Protein Interacting With Protein Kinase C Alpha 1 (PICK1) ELISA Kit
The Mouse Protein Interacting with Protein Kinase C Alpha 1 (PICK1) ELISA Kit is a reliable and sensitive tool for the accurate quantification of PICK1 levels in mouse serum, plasma, and tissue lysates. This kit offers high specificity and precision, ensuring consistent and reproducible results for a variety of research applications.PICK1 is a critical protein involved in cellular signaling pathways, particularly in the regulation of synaptic plasticity and neurotransmitter release.
Dysregulation of PICK1 has been linked to various neurological disorders, including schizophrenia and Alzheimer's disease, making it a valuable target for therapeutic research.With the Mouse PICK1 ELISA Kit, researchers can easily analyze PICK1 levels in experimental samples, providing valuable insights into the molecular mechanisms underlying neurological disorders and potential treatment strategies. Trust in the accuracy and reliability of this kit for your research needs.
Product Name:
Mouse Protein Interacting With Protein Kinase C Alpha 1 (PICK1) ELISA Kit
Product Code:
MODL01041
Size:
96 Assays
Target:
PICK1
Synonyms:
PRKCABP, Protein kinase C,Alpha Binding Protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
