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Mouse Protease Activated Receptor 2 (PAR2) ELISA Kit

SKU:
MODL01002
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P55086
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
F2RL1,F2-RL1,GPR11,PAR2,Coagulation Factor II(thrombin)receptor-Like 1,G-protein coupled receptor 11,Thrombin receptor-like 1
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Protease Activated Receptor 2 (PAR2) ELISA Kit

The Mouse Protease-Activated Receptor 2 (PAR2) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PAR2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers reliable and reproducible results, making it an essential tool for researchers studying PAR2 activation in various biological processes.PAR2 is a key receptor involved in mediating inflammation, pain, and tissue repair, making it an important target for therapeutic interventions in conditions such as inflammatory bowel disease, arthritis, and cancer.

By accurately measuring PAR2 levels, researchers can gain valuable insights into the role of this receptor in various disease states and develop targeted therapies for improved patient outcomes.Overall, the Mouse PAR2 ELISA Kit from AssayGenie provides a reliable and efficient method for quantifying PAR2 levels in mouse samples, making it ideal for a wide range of research applications in the fields of inflammation, pain, and tissue repair.

Product Name:Mouse Protease Activated Receptor 2 (PAR2) ELISA Kit
Product Code:MODL01002
Size:96 Assays
Target:PAR2
Synonyms:F2RL1, F2-RL1, GPR11, PAR2, Coagulation Factor II(thrombin)receptor-Like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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