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Mouse Proopiomelanocortin (POMC) ELISA Kit (MODL01068)

SKU:
MODL01068
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01193
Sensitivity:
0.045ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
POC,NPP,ProOMC,ACTH,Pro-OMC,Pro Opiomelanocortin,Corticotropin-lipotropin,Corticotropin,Adrenocorticotropic hormone
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Proopiomelanocortin (POMC) ELISA Kit

The Mouse Proopiomelanocortin (POMC) ELISA Kit is a reliable tool for detecting levels of POMC in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures accurate and reproducible results for a variety of research applications.POMC is a precursor protein that is processed into several biologically active peptides, including adrenocorticotropic hormone (ACTH) and beta-endorphin.

These peptides play important roles in regulating various physiological functions such as stress response, metabolism, and pain perception.Understanding the levels of POMC in mouse models can provide valuable insights into conditions related to hormone regulation, stress response, and metabolism. This ELISA kit is an essential tool for researchers studying these pathways and developing potential therapeutic interventions.

Product Name:Mouse Proopiomelanocortin (POMC) ELISA Kit
Product Code:MODL01068
Size:96 Assays
Target:POMC
Synonyms:POC, NPP, ProOMC, ACTH, Pro-OMC, Pro Opiomelanocortin, Corticotropin-lipotropin, Corticotropin, Adrenocorticotropic hormone
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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