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Mouse Prokineticin Receptor 1 (PKR1) ELISA Kit

SKU:
MODL01056
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9JKL1
Sensitivity:
0.04ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PROKR1,PKR-1,GPR73,GPR-73a,ZAQ,PK-R1,G Protein-Coupled Receptor 73,G-protein coupled receptor ZAQ
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Prokineticin Receptor 1 (PKR1) ELISA Kit

The Mouse Prokineticin Receptor 1 (PKR1) ELISA Kit is a powerful tool for detecting and quantifying levels of PKR1 in mouse samples. This kit is specifically designed for use with mouse serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for accurate results.Prokineticin receptor 1 is a key receptor involved in various physiological processes, including circadian rhythms, pain perception, and reproductive functions. Dysregulation of PKR1 has been implicated in diseases such as migraines, inflammatory conditions, and reproductive disorders, highlighting its importance as a potential therapeutic target.

With its reliable performance and ease of use, the Mouse PKR1 ELISA Kit is the perfect choice for researchers studying PKR1 signaling pathways and investigating its role in disease development. Unlock valuable insights into mouse physiology with this cutting-edge ELISA kit from Assay Genie.

Product Name:Mouse Prokineticin Receptor 1 (PKR1) ELISA Kit
Product Code:MODL01056
Size:96 Assays
Target:PKR1
Synonyms:PROKR1, PKR-1, GPR73, GPR-73a, ZAQ, PK-R1, G Protein-Coupled Receptor 73, G-protein coupled receptor ZAQ
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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