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Mouse Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

SKU:
MODL01045
Product Type:
ELISA Kit
Sensitivity:
0.195ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
P2NP,N-Propeptide Of Type II Procollagen,Procollagen II Amino Terminal Propeptide
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit

The Mouse Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit is a highly sensitive and specific assay designed for the accurate measurement of PIINP levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.PIINP is a key marker of collagen type II biosynthesis, essential for maintaining the structural integrity of connective tissues, particularly in cartilage.

Monitoring PIINP levels can provide valuable insights into the pathogenesis of diseases such as osteoarthritis and rheumatoid arthritis, as well as evaluating the effectiveness of potential therapeutic interventions.With its robust performance and ease of use, the Mouse Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit is a valuable tool for researchers studying musculoskeletal disorders and seeking to advance our understanding of collagen metabolism.

Product Name:Mouse Procollagen II N-Terminal Propeptide (PIINP) ELISA Kit
Product Code:MODL01045
Size:96 Assays
Target:PIINP
Synonyms:P2NP, N-Propeptide Of Type II Procollagen, Procollagen II Amino Terminal Propeptide
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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