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Mouse Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit

SKU:
MODL01010
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q61398
Sensitivity:
0.18ng/mL
Range:
0.468-30ng/mL
ELISA Type:
Sandwich
Synonyms:
PCOLCE,PCPE,Procollagen Type 1 COOH-Terminal Proteinase Enhancer,Procollagen C Endopeptidase Enhancer,Type I procollagen COOH-terminal proteinase enhancer
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit

The Mouse Procollagen C-Proteinase Enhancer-1 (PCPE1) ELISA Kit is specifically designed for the accurate detection of PCPE1 levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.PCPE1 is a key protein involved in collagen synthesis and regulation, playing a crucial role in maintaining connective tissue integrity and function.

Dysregulation of PCPE1 has been linked to various diseases such as fibrosis, arthritis, and skin disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic strategies.With its user-friendly protocol and quick turnaround time, the Mouse PCPE1 ELISA Kit is an essential tool for researchers looking to investigate the role of PCPE1 in health and disease in mouse models.

Product Name:Mouse Procollagen C Proteinase Enhancer 1 (PCPE1) ELISA Kit
Product Code:MODL01010
Size:96 Assays
Target:PCPE1
Synonyms:PCOLCE, PCPE, Procollagen Type 1 COOH-Terminal Proteinase Enhancer, Procollagen C Endopeptidase Enhancer, Type I procollagen COOH-terminal proteinase enhancer
Detection Method:Sandwich
Range:0.468-30ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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