The Mouse PRF1 (Perforin 1) CLIA Kit is a high-performance assay designed for the accurate detection of perforin 1 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for various research applications.Perforin 1 is a key protein involved in the immune response, particularly in the cytotoxic activity of T lymphocytes and natural killer cells. Its role in inducing cell death makes it an important factor in immune surveillance and defense against infections and tumors.
Studying perforin 1 levels can provide valuable insights into immune function and inflammatory conditions, with implications for autoimmune diseases, viral infections, and cancer research.Overall, the Mouse PRF1 (Perforin 1) CLIA Kit is a valuable tool for researchers investigating the immune system and its role in different disease processes, offering reliable and accurate measurements of perforin 1 levels in mouse samples.
Product Name:
Mouse PRF1 (Perforin 1) CLIA Kit
SKU:
MOES00470
Target:
Mouse PRF1 (Perforin 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PRF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PRF1. You can calculate the concentration of Mouse PRF1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-109
94-111
91-107
Average (%)
99
102
98
1:4
Range (%)
94-107
98-110
87-98
Average (%)
99
104
93
1:8
Range (%)
85-96
86-100
97-110
Average (%)
91
91
104
1:16
Range (%)
84-96
95-109
91-101
Average (%)
91
101
96
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-105
97
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
95-109
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.74
255.84
848.11
104.58
231.41
792.26
Standard deviation
11.73
30.34
75.14
11.24
23.72
64.81
C V (%)
10.69
11.86
8.86
10.75
10.25
8.18
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PRF1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PRF1 in samples. No significant cross-reactivity or interference between Mouse PRF1 and analogues was observed.
