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Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

SKU:
MODL01001
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8R4K8
Sensitivity:
0.065ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PAPP-A,PAPA,ASBABP2,DIPLA1,IGFBP-4ase,PAPPA1,Pappalysin 1,Insulin-Like Growth Factor Binding Protein-4 Protease,Differentially Placenta 1 Expressed Protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit

The Mouse Pregnancy-Associated Plasma Protein-A (PAPP-A) ELISA Kit is specifically designed for the accurate measurement of PAPP-A levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PAPP-A is a key protein involved in regulating insulin-like growth factor signaling and is essential for normal fetal development and pregnancy maintenance in mice.

Abnormal levels of PAPP-A have been linked to various reproductive and developmental disorders, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Mouse PAPP-A ELISA Kit provides researchers with a reliable tool for investigating the role of PAPP-A in mouse physiology and pathology, ultimately advancing our understanding of its biological functions and potential clinical implications.

Product Name:Mouse Pregnancy Associated Plasma Protein A (PAPPA) ELISA Kit
Product Code:MODL01001
Size:96 Assays
Target:PAPPA
Synonyms:PAPP-A, PAPA, ASBABP2, DIPLA1, IGFBP-4ase, PAPPA1, Pappalysin 1, Insulin-Like Growth Factor Binding Protein-4 Protease, Differentially Placenta 1 Expressed Protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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