The Mouse PPAR Gamma (Peroxisome Proliferator-Activated Receptor Gamma) ELISA Kit from Assay Genie is specifically designed for the accurate measurement of PPAR Gamma levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, allowing for reliable and reproducible results that are essential for a wide range of research applications.PPAR Gamma is a critical transcription factor involved in regulating lipid and glucose metabolism, making it a key player in various metabolic disorders such as obesity, diabetes, and cardiovascular diseases.
By accurately measuring PPAR Gamma levels, researchers can gain insights into its involvement in these conditions and potentially identify novel therapeutic targets.Overall, the Mouse PPAR Gamma ELISA Kit offers researchers a powerful tool for studying the role of PPAR Gamma in metabolic disorders and developing innovative treatment strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PPAR-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PPAR-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PPAR-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PPAR-γ. You can calculate the concentration of Mouse PPAR-γ in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-95
84-97
93-108
Average (%)
90
91
101
1:4
Range (%)
91-104
82-94
84-99
Average (%)
98
88
90
1:8
Range (%)
87-102
82-93
88-101
Average (%)
93
87
93
1:16
Range (%)
92-106
84-98
81-93
Average (%)
99
90
87
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-98
91
EDTA plasma (n=5)
89-105
96
Cell culture media (n=5)
88-101
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.55
1.57
4.57
0.56
1.43
4.45
Standard deviation
0.03
0.08
0.14
0.04
0.06
0.2
C V (%)
5.45
5.1
3.06
7.14
4.2
4.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PPAR-γ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PPAR-γ in samples. No significant cross-reactivity or interference between Mouse PPAR-γ and analogues was observed.
