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Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

SKU:
MODL01043
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O70570
Sensitivity:
1.34ng/mL
Range:
3.125-200ng/mL
ELISA Type:
Sandwich
Synonyms:
SC,Secretory Component,Hepatocellular carcinoma-associated protein TB6
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit

The Mouse Polymeric Immunoglobulin Receptor (pIgR) ELISA Kit is specifically designed for the accurate and precise measurement of pIgR levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The polymeric immunoglobulin receptor (pIgR) is a key protein involved in the transport of polymeric immunoglobulins across mucosal epithelial cells, playing a crucial role in mucosal immunity and gut health.

Dysregulation of pIgR has been implicated in various inflammatory and autoimmune diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and high-quality components, the Mouse Polymeric Immunoglobulin Receptor (pIgR) ELISA Kit is a valuable tool for researchers looking to explore the role of pIgR in immune responses and mucosal immunity in mouse models.

Product Name:Mouse Polymeric Immunoglobulin Receptor (PIGR) ELISA Kit
Product Code:MODL01043
Size:96 Assays
Target:PIGR
Synonyms:SC, Secretory Component, Hepatocellular carcinoma-associated protein TB6
Detection Method:Sandwich
Range:3.12-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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