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Mouse Platelet Membrane Glycoprotein IV (GP4) ELISA Kit

SKU:
MODL00568
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q08857
Sensitivity:
0.18ng/mL
Range:
0.39-25ng/mL
ELISA Type:
Sandwich
Synonyms:
443.3,CD36,FAT,GP3B,GPIV,PASIV,SCARB3,GP88,GpIIIb,Glycoprotein IIIb,Glycoprotein IIIb,Thrombospondin Receptor,Fatty acid translocase,Platelet collagen receptor
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

Mouse Platelet Membrane Glycoprotein IV (GP4) ELISA Kit

The Mouse Platelet Membrane Glycoprotein IV (GP4) ELISA Kit is a highly reliable and accurate tool for the measurement of GP4 levels in mouse samples. This kit is specifically designed for use in serum, plasma, and cell culture supernatants, providing researchers with precise and reproducible results.GP4 is an important membrane glycoprotein involved in platelet function and adhesion, playing a crucial role in hemostasis and thrombosis. Its levels have been implicated in various diseases and conditions such as bleeding disorders and cardiovascular diseases, making it a valuable biomarker for research and clinical studies.

With its high sensitivity and specificity, the Mouse Platelet Membrane Glycoprotein IV (GP4) ELISA Kit is an essential tool for studying the role of GP4 in physiological and pathological processes, as well as for developing potential therapeutic interventions.

Product Name:Mouse Platelet Membrane Glycoprotein IV (GP4) ELISA Kit
Product Code:MODL00568
Size:96 Assays
Target:GP4
Synonyms:CD36, FAT, GP3B, GPIV, PASIV, SCARB3, GP88, GpIIIb, Glycoprotein IIIb, Collagen Type I Receptor, Thrombospondin Receptor, Fatty acid translocase, Platelet collagen
Detection Method:Sandwich
Range:0.39-25ng/ml
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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