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Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

SKU:
MODL01020
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31240
Sensitivity:
11.2pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDGF-B,PDGF2,SIS,SSV,C-sis,Simian Sarcoma Viral(v-sis)Oncogene Homolog,Platelet Derived Growth Factor Beta Polypeptide,Proto-oncogene c-Sis,Becaplermin
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit

The Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit is specifically designed for the accurate measurement of PDGFB levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.PDGFB is a key growth factor involved in cell proliferation, migration, and differentiation, playing a crucial role in processes such as wound healing and tissue repair.

Dysregulation of PDGFB has been linked to various diseases, including cancers and fibrotic disorders, making it a valuable biomarker for investigating these conditions and potential therapeutic interventions.Overall, the Mouse PDGFB ELISA Kit provides reliable and efficient detection of PDGFB levels in biological samples, making it an essential tool for researchers studying the role of PDGFB in health and disease.

Product Name:Mouse Platelet Derived Growth Factor Subunit B (PDGFB) ELISA Kit
Product Code:MODL01020
Size:96 Assays
Target:PDGFB
Synonyms:PDGF-B, PDGF2, SIS, SSV, C-sis, Simian Sarcoma Viral(v-sis)Oncogene Homolog, Platelet Derived Growth Factor Beta Polypeptide, Proto-oncogene c-Sis, Becaplermin
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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