null

Mouse Platelet Derived Growth Factor C (PDGFC) ELISA Kit

SKU:
MODL01021
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8CI19
Sensitivity:
13.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
PDGF-C,SCDGF,VEGF-E,Fallotein,Spinal cord-derived growth factor,Platelet-derived growth factor C, latent form,Platelet-derived growth factor C, receptor-binding form
Reactivity:
Mouse
Sample Type:
Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Platelet Derived Growth Factor C (PDGFC) ELISA Kit

The Mouse Platelet-Derived Growth Factor C (PDGFC) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PDGFC levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it perfect for a variety of research applications.PDGFC is a key growth factor involved in cell proliferation, migration, and differentiation, playing a crucial role in wound healing, tissue repair, and cardiovascular development.

Its dysregulation has been implicated in various diseases, including cancer and fibrotic disorders, making it an important target for therapeutic interventions.With its high performance and versatility, the Mouse PDGFC ELISA Kit is an essential tool for researchers studying the biological functions and therapeutic potential of PDGFC, advancing our understanding of its role in health and disease.

Product Name:Mouse Platelet Derived Growth Factor C (PDGFC) ELISA Kit
Product Code:MODL01021
Size:96 Assays
Target:PDGFC
Synonyms:PDGF-C, SCDGF, VEGF-E, Fallotein, Spinal cord-derived growth factor, Platelet-derived growth factor C, latent form, Platelet-derived growth factor C, receptor-binding form
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products