null

Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit

SKU:
MODL00997
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8VDG7
Sensitivity:
0.062ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SD-PLA2,Serine-dependent phospholipase A2,Platelet-activating factor acetylhydrolase 2, cytoplasmic
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit

The Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit is specifically designed for the accurate measurement of PAFAH2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.PAFAH2 is an important enzyme that plays a critical role in the metabolism of platelet activating factor (PAF), a potent lipid mediator involved in inflammation, immune response, and various physiological processes.

Dysregulation of PAFAH2 has been associated with inflammatory diseases, neurological disorders, and cancer, highlighting its significance as a potential biomarker for studying these conditions and developing therapeutic interventions.With the Mouse PAFAH2 ELISA Kit, researchers can accurately quantify PAFAH2 levels in mouse samples, allowing for detailed investigations into the role of this enzyme in health and disease. Trust in the reliability and accuracy of this kit for your research needs.

Product Name:Mouse Platelet Activating Factor Acetylhydrolase 2 (PAFAH2) ELISA Kit
Product Code:MODL00997
Size:96 Assays
Target:PAFAH2
Synonyms:SD-PLA2, Serine-dependent phospholipase A2, Platelet-activating factor acetylhydrolase 2, cytoplasmic
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products