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Mouse Plasminogen Activator, Urokinase (uPA) ELISA Kit

SKU:
MODL01361
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06869
Sensitivity:
4.1pg/mL
Range:
9.375-600pg/mL
ELISA Type:
Sandwich
Synonyms:
PLAU,ATF,URK,UK,UP-A,Abbokinase,Urokinase-Type Plasminogen Activator
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Plasminogen Activator, Urokinase (uPA) ELISA Kit

The Mouse Plasminogen Activator Urokinase (UPA) ELISA Kit is specifically designed for the accurate measurement of UPA levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.UPA is an important enzyme involved in the regulation of the fibrinolytic system, playing a crucial role in processes like clot formation and tissue remodeling.

Dysregulation of UPA activity has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic strategies.With its reliable performance and ease of use, the Mouse Plasminogen Activator Urokinase (UPA) ELISA Kit is an essential tool for researchers looking to investigate the role of UPA in health and disease in mouse models.

Product Name:Mouse Plasminogen Activator, Urokinase (uPA) ELISA Kit
Product Code:MODL01361
Size:96 Assays
Target:uPA
Synonyms:PLAU, ATF, URK, UK, UP-A, Abbokinase, Urokinase-Type Plasminogen Activator
Detection Method:Sandwich
Range:9.375-600pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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