null

Mouse Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit

SKU:
MODL01362
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35456
Sensitivity:
47pg/mL
Range:
125-8000pg/mL
ELISA Type:
Sandwich
Synonyms:
CD87,PLAUR,PLAU-R,U-PAR,URKR,Monocyte Activation Antigen Mo3
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit

The Mouse Plasminogen Activator Urokinase Receptor (uPAR) ELISA Kit is specifically designed for the precise measurement of uPAR levels in mouse serum, plasma, and tissue lysates. This kit offers exceptional sensitivity and accuracy, guaranteeing dependable and consistent results for various research purposes.The uPAR protein is a key component in the regulation of the plasminogen activation system, essential for processes like cell migration, invasion, and adhesion.

It plays a crucial role in various diseases, including cancer, inflammation, and cardiovascular disorders, making it a valuable biomarker for investigating these conditions and developing potential therapeutic interventions.Overall, the Mouse Plasminogen Activator Urokinase Receptor (uPAR) ELISA Kit is an indispensable tool for researchers looking to deepen their understanding of uPAR biology and its implications in various pathological conditions.

Product Name:Mouse Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Product Code:MODL01362
Size:96 Assays
Target:uPAR
Synonyms:CD87, PLAUR, PLAU-R, U-PAR, URKR, Monocyte Activation Antigen Mo3
Detection Method:Sandwich
Range:125-8,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products