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Mouse Plasminogen Activator Inhibitor 2 (PAI2) ELISA Kit

SKU:
MODL00999
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12388
Sensitivity:
0.134ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SERPINB2,PLANH2,Serpin Peptidase Inhibitor Clade B Member 2,Monocyte Arg-serpin,Placental plasminogen activator inhibitor,Serpin B2,Urokinase inhibitor
Reactivity:
Mouse
Sample Type:
Plasma
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Plasminogen Activator Inhibitor 2 (PAI2) ELISA Kit

The Mouse Plasminogen Activator Inhibitor-2 (PAI-2) ELISA Kit is a powerful tool for measuring levels of PAI-2 in mouse serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, providing accurate and reliable results for a variety of research applications.PAI-2 is an important protein involved in regulating the fibrinolytic system and has implications in various diseases, such as cancer, cardiovascular diseases, and inflammation.

By accurately measuring PAI-2 levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.With its ease of use and robust performance, the Mouse PAI-2 ELISA Kit is a valuable resource for researchers studying the role of PAI-2 in health and disease. Get precise and reproducible results with this advanced ELISA kit from Assay Genie.

Product Name:Mouse Plasminogen Activator Inhibitor 2 (PAI2) ELISA Kit
Product Code:MODL00999
Size:96 Assays
Target:PAI2
Synonyms:SERPINB2, PLANH2, Serpin Peptidase Inhibitor Clade B Member 2, Monocyte Arg-serpin, Placental plasminogen activator inhibitor, Serpin B2, Urokinase inhibitor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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