The Mouse PKR1 (Prokineticin Receptor 1) CLIA Kit is a highly accurate and sensitive assay designed for the quantitative detection of Prokineticin Receptor 1 levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Prokineticin Receptor 1 is a key receptor involved in various physiological processes, including circadian rhythms, pain perception, and neurogenesis.
Dysregulation of PKR1 has been implicated in conditions such as migraine, inflammation, and psychiatric disorders, making it a valuable biomarker for studying these conditions and developing potential therapies.Overall, the Mouse PKR1 CLIA Kit offers researchers a powerful tool for investigating the role of Prokineticin Receptor 1 in health and disease, providing valuable insights into its functions and potential therapeutic applications.
Product Name:
Mouse PKR1 (Prokineticin Receptor 1) CLIA Kit
SKU:
MOES00497
Target:
Mouse PKR1 (Prokineticin Receptor 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PKR1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PKR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PKR1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PKR1. You can calculate the concentration of Mouse PKR1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-111
86-102
98-110
Average (%)
102
93
103
1:4
Range (%)
96-114
87-100
92-105
Average (%)
104
92
98
1:8
Range (%)
96-112
99-113
104-117
Average (%)
102
107
110
1:16
Range (%)
91-103
88-99
99-118
Average (%)
98
93
107
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-107
100
EDTA plasma (n=5)
95-110
102
Cell culture media (n=5)
88-98
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
93.59
207.03
727.39
100.35
191.28
662.54
Standard deviation
8.33
19.3
50.48
8.8
18.76
53.53
C V (%)
8.9
9.32
6.94
8.77
9.81
8.08
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PKR1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PKR1 in samples. No significant cross-reactivity or interference between Mouse PKR1 and analogues was observed.
