The Mouse PI (proinsulin) CLIA Kit is a reliable and accurate tool for measuring proinsulin levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring consistent and reproducible results for various research applications.Proinsulin is a key protein involved in insulin production and regulation, making it a valuable biomarker for studying diabetes, metabolic disorders, and other related conditions.
By accurately measuring proinsulin levels, researchers can gain valuable insights into disease progression and potential therapeutic targets.Overall, the Mouse PI CLIA Kit is an essential tool for researchers looking to study proinsulin and its role in various physiological and pathological processes in mouse models. Its high-quality performance and ease of use make it a valuable addition to any laboratory setting.
Product Name:
Mouse PI (Proinsulin) CLIA Kit
SKU:
MOES00495
Target:
Mouse PI (Proinsulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse PI. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PI, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse PI. You can calculate the concentration of Mouse PI in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-118
91-107
86-98
Average (%)
109
98
91
1:4
Range (%)
89-102
85-97
102-116
Average (%)
94
91
108
1:8
Range (%)
101-118
92-106
93-105
Average (%)
108
99
100
1:16
Range (%)
85-98
90-102
99-110
Average (%)
91
97
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-116
107
EDTA plasma (n=5)
93-111
101
Cell culture media (n=5)
93-105
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
97.88
315.02
671.24
101.21
326.02
693.68
Standard deviation
10.01
28.07
59.61
9.61
24.22
69.58
C V (%)
10.23
8.91
8.88
9.5
7.43
10.03
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse PI concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PI in samples. No significant cross-reactivity or interference between Mouse PI and analogues was observed.
