null

Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

SKU:
MODL01058
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31482
Sensitivity:
5.7pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
PLA2,MOM1,PLA2B,PLA2L,PLA2S,PLAS1,sPLA2(platelets,synovial fluid),Non-pancreatic secretory phospholipase A2,Phosphatidylcholine 2-acylhydrolase 2A
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit

The Mouse Phospholipase A2 Group IIA (PLA2G2A) ELISA Kit is a high-quality assay designed for the accurate measurement of PLA2G2A levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PLA2G2A is an important enzyme involved in the metabolism of phospholipids and the inflammatory response. It plays a key role in various physiological processes and has been implicated in inflammatory diseases, cardiovascular disorders, and cancer.

The accurate measurement of PLA2G2A levels is essential for studying its functions, identifying potential therapeutic targets, and developing new treatments.Overall, the Mouse Phospholipase A2 Group IIA (PLA2G2A) ELISA Kit is a valuable tool for researchers studying the role of PLA2G2A in health and disease, providing reliable data for further investigation and discovery.

Product Name:Mouse Phospholipase A2, Group IIA (PLA2G2A) ELISA Kit
Product Code:MODL01058
Size:96 Assays
Target:PLA2G2A
Synonyms:PLA2, MOM1, PLA2B, PLA2L, PLA2S, PLAS1, sPLA2(platelets,synovial fluid), Non-pancreatic secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 2A
Detection Method:Sandwich
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products