null

Mouse Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit

SKU:
MODL01046
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q61194
Sensitivity:
0.057ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
PI3K2a,CPK,PI3-K-C2(Alpha),PI3-K-C2A,PI3K2-a,Phosphoinositide 3-Kinase 2a,Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit

The Mouse Phosphoinositide 3-kinase Class 2 Alpha Polypeptide (PIK3C2A) ELISA Kit is a powerful tool for accurately measuring levels of PIK3C2A in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.PIK3C2A is a key enzyme belonging to the PI3K family, playing a crucial role in cell signaling pathways related to cell growth, survival, and metabolism.

Dysregulation of PIK3C2A has been implicated in various diseases, including cancer, diabetes, and autoimmune disorders, making it a valuable target for therapeutic interventions and biomarker studies.By utilizing the Mouse PIK3C2A ELISA Kit, researchers can gain valuable insights into the molecular mechanisms underlying disease pathogenesis and potential therapeutic strategies, ultimately advancing our understanding of complex biological processes.

Product Name:Mouse Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide (PIK3C2a) ELISA Kit
Product Code:MODL01046
Size:96 Assays
Target:PIK3C2a
Synonyms:PI3K2a, CPK, PI3-K-C2(Alpha), PI3-K-C2A, PI3K2-a, Phosphoinositide 3-Kinase 2a, Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products