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Mouse Phosphodiesterase 12 (PDE12) ELISA Kit

SKU:
MODL01015
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3TIU4
Sensitivity:
0.051ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
2'-PDE,2'-Phosphodiesterase,Mitochondrial deadenylase
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Phosphodiesterase 12 (PDE12) ELISA Kit

The Mouse Phosphodiesterase 12 (PDE12) ELISA Kit is a reliable tool for the quantitative detection of PDE12 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.PDE12 is an enzyme involved in the regulation of cyclic nucleotide signaling and has been implicated in various cellular processes, including cell growth, differentiation, and apoptosis.

Dysregulation of PDE12 has been associated with a range of diseases, such as cancer, inflammation, and neurological disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse Phosphodiesterase 12 (PDE12) ELISA Kit provides researchers with a powerful tool for studying the role of PDE12 in health and disease, offering valuable insights into potential treatment strategies and drug development.

Product Name:Mouse Phosphodiesterase 12 (PDE12) ELISA Kit
Product Code:MODL01015
Size:96 Assays
Target:PDE12
Synonyms:2'-PDE, 2'-Phosphodiesterase, Mitochondrial deadenylase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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