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Mouse Phenol Sulfotransferase (PST) ELISA Kit

SKU:
MODL01097
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q91W19
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SULT1A1,HAST1/HAST2,ST1A3,STP1,TSPST1,Sulfotransferase 1A1,Aryl sulfotransferase 1,Phenol sulfotransferase 1,Thermostable phenol sulfotransferase
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

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Mouse Phenol Sulfotransferase (PST) ELISA Kit

The Mouse Phenol Sulfotransferase (PST) ELISA Kit is a powerful tool for accurately measuring phenol sulfotransferase levels in mouse samples. This kit offers exceptional sensitivity and specificity, ensuring trustworthy and consistent results for a variety of research purposes.Phenol sulfotransferase is a key enzyme involved in the metabolism of various drugs, hormones, and environmental chemicals in mice. Its activity can impact drug efficacy and toxicity, making it a valuable target for drug development and toxicity studies.

By using the Mouse PST ELISA Kit, researchers can gain valuable insights into the role of phenol sulfotransferase in drug metabolism and potential drug interactions in mouse models. This kit is essential for studying the impact of phenol sulfotransferase on drug efficacy and toxicity, making it a vital tool for drug development and toxicology research in mice.

Product Name:Mouse Phenol Sulfotransferase (PST) ELISA Kit
Product Code:MODL01097
Size:96 Assays
Target:PST
Synonyms:SULT1A1, HAST1/HAST2, ST1A3, STP1, TSPST1, Sulfotransferase 1A1, Aryl sulfotransferase 1, Phenol sulfotransferase 1, Thermostable phenol sulfotransferase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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