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Mouse PHD Finger Protein 8 (PHF8) ELISA Kit

SKU:
MODL01040
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q80TJ7
Sensitivity:
0.118ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ZNF422,MRXSSD,JHDM1F,Jumonji C Domain-Containing Histone Demethylase 1F,Histone lysine demethylase PHF8
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Mouse PHD Finger Protein 8 (PHF8) ELISA Kit

The Mouse PHD Finger Protein 8 (PHF8) ELISA Kit is a reliable and sensitive tool for the precise detection of PHF8 levels in mouse samples, including serum, plasma, and cell culture supernatants. This kit offers high specificity and accuracy, ensuring trustworthy and consistent results for various research applications.PHF8 is a key protein involved in epigenetic regulation, playing an essential role in gene expression and cell differentiation.

Dysregulation of PHF8 has been linked to various diseases, including cancer and neurodevelopmental disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.Overall, the Mouse PHF8 ELISA Kit is a valuable tool for researchers looking to investigate the role of PHF8 in disease pathogenesis and explore its potential as a target for novel treatments.

Product Name:Mouse PHD Finger Protein 8 (PHF8) ELISA Kit
Product Code:MODL01040
Size:96 Assays
Target:PHF8
Synonyms:ZNF422, MRXSSD, JHDM1F, Jumonji C Domain-Containing Histone Demethylase 1F, Histone lysine demethylase PHF8
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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