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Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit

SKU:
MODL01074
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35396
Sensitivity:
0.052ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
FAAR,NR1C2,NUC1,NUCI,PPARB,PPARd/B,Nuclear Receptor Subfamily 1,Group C,Member 2,Nuclear hormone receptor 1,Peroxisome proliferator-activated receptor beta
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit

The Mouse Peroxisome Proliferator-Activated Receptor Delta (PPARD) ELISA Kit is a reliable and precise tool for measuring levels of PPARD in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it ideal for various research applications.PPARD is a key regulator of lipid metabolism and inflammation, playing a crucial role in metabolic diseases such as obesity and diabetes.

By detecting and quantifying PPARD levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets.Overall, the Mouse PPARD ELISA Kit is a valuable resource for studying the role of PPARD in metabolic disorders and developing targeted therapies to improve human health.

Product Name:Mouse Peroxisome Proliferator Activated Receptor Delta (PPARd) ELISA Kit
Product Code:MODL01074
Size:96 Assays
Target:PPARd
Synonyms:FAAR, NR1C2, NUC1, NUCI, PPARB, PPARd/B, Nuclear Receptor Subfamily 1,Group C,Member 2, Nuclear hormone receptor 1, Peroxisome proliferator-activated receptor beta
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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