The Mouse Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit is a sophisticated tool designed for the precise quantitative detection of PAI1 levels in various biological samples. PAI1 is a key regulator of the plasminogen activation system, playing a critical role in controlling fibrinolysis and coagulation.
Aberrant expression of PAI1 has been associated with various cardiovascular diseases, thrombotic disorders, and fibrotic conditions. Accurate measurement of PAI1 levels using this ELISA kit is essential for gaining insights into the pathophysiological processes involving fibrinolysis, clot formation, and tissue remodeling. Researchers can utilize this kit to explore the role of PAI1 in thrombotic events, atherosclerosis, and other related conditions. With exceptional sensitivity and specificity, the PAI1 ELISA kit from Assay Genie ensures reliable and reproducible results, enabling researchers to delve deeper into the mechanisms underlying vascular disorders and thrombotic diseases. Manufactured under stringent quality control standards, this kit offers robust performance and user-friendly protocols, making it an excellent choice for cutting-edge research in the fields of coagulation biology and cardiovascular health.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PAI1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PAI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse PAI1. You can calculate the concentration of Mouse PAI1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
83-94
86-99
93-102
Average (%)
91
92
97
1:4
Range (%)
98-107
91-98
87-97
Average (%)
101
94
93
1:8
Range (%)
88-97
85-98
86-97
Average (%)
91
91
94
1:16
Range (%)
98-105
99-109
95-111
Average (%)
102
105
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-102
97
EDTA plasma (n=5)
98-107
104
Cell culture media (n=5)
101-109
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.02
5.24
22.99
2.31
6.05
24.13
Standard deviation
0.11
0.24
1.07
0.12
0.28
1.3
C V (%)
5.45
4.58
4.65
5.19
4.63
5.39
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse PAI1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse PAI1 in samples. No significant cross-reactivity or interference between Mouse PAI1 and analogues was observed.
