Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
The Assay Genie Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit is meticulously crafted to facilitate the precise and quantitative measurement of Oxidized Low-Density Lipoprotein (OxLDL) levels within diverse biological samples sourced from mouse models. OxLDL is a pivotal marker associated with oxidative stress, atherosclerosis, and cardiovascular diseases, making accurate monitoring of its concentrations integral to studying lipid metabolism, vascular health, and inflammatory processes. Our Mouse OxLDL ELISA Kit is designed to deliver exceptional sensitivity and specificity, ensuring the generation of reliable and reproducible results crucial for assessing OxLDL's involvement in disease states and potential therapeutic strategies.
Manufactured under rigorous quality control measures, this kit ensures robust performance while maintaining user-friendly protocols, making it an optimal choice for research applications focused on investigating the role of Oxidized Low-Density Lipoprotein in mouse models. Trust the Assay Genie Mouse OxLDL ELISA Kit for accurate and dependable quantification of this key biomarker in your experimental investigations.
Product Name:
Mouse OxLDL (Oxidized Low Density Lipoprotein) ELISA Kit
SKU:
AEES00187
Target:
Mouse OxLDL (Oxidized Low Density Lipoprotein)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OxLDL. You can calculate the concentration of Mouse OxLDL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
94-105
95-108
Average (%)
99
99
102
1:4
Range (%)
88-100
85-101
92-102
Average (%)
94
93
96
1:8
Range (%)
87-100
94-102
96-108
Average (%)
93
98
103
1:16
Range (%)
87-98
93-102
99-112
Average (%)
92
97
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
91
EDTA plasma (n=5)
94-104
100
Cell culture media (n=5)
92-102
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.95
2.36
8.45
0.85
2.15
8.82
Standard deviation
0.05
0.11
0.45
0.05
0.1
0.39
C V (%)
5.26
4.66
5.33
5.88
4.65
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse OxLDL concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OxLDL in samples. No significant cross-reactivity or interference between Mouse OxLDL and analogues was observed.
