The Mouse Osteopontin (OPN) ELISA Kit is meticulously designed to quantitatively detect Osteopontin levels in various mouse biological samples. Osteopontin, a multifunctional protein, is involved in several cellular processes including bone mineralization, immune responses, and inflammation modulation. This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results in research investigations related to bone metabolism, immune regulation, and inflammatory conditions.
Manufactured under stringent quality control measures, this kit provides reliable performance, making it an ideal choice for impactful scientific research.
Product Name:
Mouse OPN (Osteopontin) ELISA Kit
SKU:
AEES00225
Target:
Mouse OPN (Osteopontin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse OPN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OPN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OPN, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse OPN. You can calculate the concentration of Mouse OPN in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-110
97-108
95-105
Average (%)
105
101
102
1:4
Range (%)
97-108
87-97
84-97
Average (%)
105
91
90
1:8
Range (%)
96-104
91-103
94-105
Average (%)
99
95
102
1:16
Range (%)
95-104
91-100
91-105
Average (%)
98
97
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-97
92
EDTA plasma (n=5)
101-111
104
Cell culture media (n=5)
84-94
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.21
4.13
0.45
1.08
4.22
Standard deviation
0.03
0.06
0.22
0.02
0.05
0.21
C V (%)
6
4.96
5.33
4.44
4.63
4.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse OPN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OPN in samples. No significant cross-reactivity or interference between Mouse OPN and analogues was observed.
