The Mouse Olfm4 (Olfactomedin 4) CLIA Kit is a powerful tool for measuring Olfm4 levels in mouse samples with high accuracy and precision. This kit has been carefully optimized for detecting Olfm4 in serum, plasma, and tissue culture media, providing researchers with a reliable and reproducible assay for their studies.Olfm4, also known as GW112 or hGC-1, is a glycoprotein involved in stem cell self-renewal, differentiation, and tissue regeneration. It has been implicated in various biological processes, including immune response regulation, cancer development, and inflammatory diseases.
The measurement of Olfm4 levels can offer valuable insights into these processes and potentially serve as a biomarker for disease diagnosis and monitoring.Overall, the Mouse Olfm4 CLIA Kit offers a comprehensive and efficient solution for studying the role of Olfm4 in mouse models, advancing our understanding of its functions and implications in health and disease.
Product Name:
Mouse OLFM4 (Olfactomedin 4) CLIA Kit
SKU:
MOES00459
Target:
Mouse OLFM4 (Olfactomedin 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse OLFM4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse OLFM4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse OLFM4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse OLFM4. You can calculate the concentration of Mouse OLFM4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
99-117
101-117
Average (%)
90
108
109
1:4
Range (%)
91-103
89-99
99-115
Average (%)
97
94
105
1:8
Range (%)
87-101
101-118
101-112
Average (%)
93
108
107
1:16
Range (%)
92-104
99-111
103-119
Average (%)
98
104
110
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-109
99
EDTA plasma (n=5)
90-104
98
Cell culture media (n=5)
89-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
36.97
107.37
326.8
38.19
99.02
304.86
Standard deviation
3.23
9.55
30.56
4.46
11.57
25.33
C V (%)
8.74
8.89
9.35
11.68
11.68
8.31
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse OLFM4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse OLFM4 in samples. No significant cross-reactivity or interference between Mouse OLFM4 and analogues was observed.
