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Mouse Olfactomedin 4 (OLFM4) ELISA Kit

SKU:
MODL00987
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
D3YYD0
Sensitivity:
13.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
OLM4,hGC1,hOLfD,OlfD,GW112,GC1,G-CSF-stimulated clone 1 protein,Antiapoptotic protein GW112
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
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Description

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Mouse Olfactomedin 4 (OLFM4) ELISA Kit

The Mouse Olfactomedin-4 (OLFM4) ELISA Kit is specifically designed for the accurate and sensitive detection of Olfactomedin-4 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for various research applications.Olfactomedin-4 is a key protein involved in various biological processes, including cell adhesion, migration, and signaling. It plays a crucial role in immune response, inflammation, and tumor progression, making it a valuable biomarker for studying these processes and developing targeted therapies.

With its easy-to-use protocol and high-quality components, the Mouse Olfactomedin-4 (OLFM4) ELISA Kit is an essential tool for researchers seeking to accurately quantify Olfactomedin-4 levels in mouse samples. Order yours today to advance your research in immunology, cancer biology, and beyond.

Product Name:Mouse Olfactomedin 4 (OLFM4) ELISA Kit
Product Code:MODL00987
Size:96 Assays
Target:OLFM4
Synonyms:OLM4, hGC1, hOLfD, OlfD, GW112, GC1, G-CSF-stimulated clone 1 protein, Antiapoptotic protein GW112
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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