null

Mouse Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit

SKU:
MODL00964
Product Type:
ELISA Kit
Sensitivity:
33.7pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
GR,GCCR,GCR,GR,GRL,NR3-C1,Glucocorticoid Receptor
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit

The Mouse Nuclear Receptor Subfamily 3 Group C Member 1 (NR3C1) ELISA Kit is specifically designed for the accurate quantification of NR3C1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NR3C1, also known as the glucocorticoid receptor, plays a crucial role in mediating the effects of glucocorticoid hormones on gene expression and cellular function.

Dysregulation of NR3C1 has been implicated in a variety of diseases and conditions, including inflammatory disorders, metabolic syndromes, and stress-related illnesses.By accurately measuring NR3C1 levels, researchers can gain valuable insights into the role of this nuclear receptor in various physiological processes and disease states. This ELISA kit provides a powerful tool for studying NR3C1 biology and developing potential therapeutic strategies for related disorders.

Product Name:Mouse Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) ELISA Kit
Product Code:MODL00964
Size:96 Assays
Target:NR3C1
Synonyms:GR, GCCR, GCR, GR, GRL, NR3-C1, Glucocorticoid Receptor
Detection Method:Sandwich
Range:78.125-5000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products