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Mouse Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit

SKU:
MODL00963
Product Type:
ELISA Kit
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HZF2,BD73,EAR-1r,RVR,Rev-ErbA Beta,Orphan nuclear hormone receptor BD73,Rev-erb alpha-related receptor,V-erbA-related protein 1-related
Reactivity:
Mouse
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit

The Mouse NR1D2 (Nuclear Receptor Subfamily 1 Group D Member 2) ELISA Kit is specifically designed for the accurate quantification of NR1D2 levels in mouse serum, plasma, and cell lysates. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.NR1D2 is a key regulator of circadian rhythm and metabolism, playing a crucial role in the regulation of genes involved in various physiological processes. Dysregulation of NR1D2 has been linked to metabolic disorders, cardiovascular diseases, and neurological conditions, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions.

With its user-friendly protocol and high-performance features, the Mouse NR1D2 ELISA Kit is an essential tool for researchers investigating the role of NR1D2 in health and disease. Order yours today to advance your research efforts in the field of circadian biology and metabolic regulation.

Product Name:Mouse Nuclear Receptor Subfamily 1, Group D, Member 2 (NR1D2) ELISA Kit
Product Code:MODL00963
Size:96 Assays
Target:NR1D2
Synonyms:HZF2, BD73, EAR-1r, RVR, Rev-ErbA Beta, Orphan nuclear hormone receptor BD73, Rev-erb alpha-related receptor, V-erbA-related protein 1-related
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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