Mouse NRIP1 (Nuclear Receptor Interacting Protein 1) CLIA Kit
The Mouse NRIP1 (Nuclear Receptor Interacting Protein 1) CLIA Kit is a reliable and accurate tool for the quantitative detection of NRIP1 levels in mouse samples such as serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides consistent and trustworthy results, making it suitable for a variety of research applications.NRIP1 is a key protein that interacts with nuclear receptors, influencing gene expression and cellular function. Dysregulation of NRIP1 has been implicated in various diseases, including cancer, metabolic disorders, and cardiovascular conditions.
Therefore, measuring NRIP1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets.With its user-friendly format and robust performance, the Mouse NRIP1 CLIA Kit is an essential tool for researchers studying the role of NRIP1 in health and disease, offering a comprehensive solution for accurate quantification and analysis.
Product Name:
Mouse NRIP1 (Nuclear Receptor Interacting Protein 1) CLIA Kit
SKU:
MOES00457
Target:
Mouse NRIP1 (Nuclear Receptor Interacting Protein 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NRIP1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NRIP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NRIP1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NRIP1. You can calculate the concentration of Mouse NRIP1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
85-97
88-102
Average (%)
96
90
95
1:4
Range (%)
88-104
86-98
100-117
Average (%)
95
92
108
1:8
Range (%)
92-106
90-105
91-106
Average (%)
100
97
97
1:16
Range (%)
93-106
90-106
96-114
Average (%)
100
96
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-108
100
EDTA plasma (n=5)
100-115
106
Cell culture media (n=5)
90-103
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.47
4.29
23.62
2.71
4.26
22.38
Standard deviation
0.31
0.45
2.64
0.32
0.46
2.38
C V (%)
12.55
10.49
11.18
11.81
10.8
10.63
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NRIP1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NRIP1 in samples. No significant cross-reactivity or interference between Mouse NRIP1 and analogues was observed.
