The Mouse NOS3 (eNOS) Nitric Oxide Synthase 3 (Endothelial) CLIA Kit is a powerful tool for accurately measuring the levels of nitric oxide synthase 3 in mouse samples. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for various research applications.Nitric oxide synthase 3 (eNOS) is a key enzyme involved in the production of nitric oxide, a crucial signaling molecule that plays a vital role in various physiological processes, including regulation of blood flow, immune response, and neurotransmission.
Dysregulation of eNOS activity has been linked to numerous health conditions, such as hypertension, atherosclerosis, and diabetes, highlighting its importance as a potential therapeutic target.With the Mouse NOS3 (eNOS) Nitric Oxide Synthase 3 (Endothelial) CLIA Kit, researchers can gain valuable insights into the role of eNOS in health and disease, paving the way for the development of novel treatment strategies and diagnostic tools.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS3/eNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NOS3/eNOS. You can calculate the concentration of Mouse NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-112
95-109
93-108
Average (%)
103
102
99
1:4
Range (%)
88-104
93-107
85-98
Average (%)
96
99
92
1:8
Range (%)
86-99
92-107
91-104
Average (%)
93
98
97
1:16
Range (%)
97-112
102-115
92-106
Average (%)
104
108
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
101
EDTA plasma (n=5)
85-98
90
Cell culture media (n=5)
92-110
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.97
79.34
182.35
25.49
73.64
187.28
Standard deviation
2.53
5.62
14.19
3.07
7.74
16.87
C V (%)
10.55
7.08
7.78
12.04
10.51
9.01
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NOS3/eNOS concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
