The Mouse NOS1 (NNOS) Nitric Oxide Synthase 1 Neuronal CLIA Kit is a reliable and accurate tool for the quantitative measurement of NOS1 levels in mouse serum, plasma, and tissue lysates. This kit is highly sensitive and specific, allowing for precise and reproducible results in various research applications.Nitric oxide synthase 1 (NOS1), also known as neuronal NOS (nNOS), plays a critical role in the production of nitric oxide, a key signaling molecule involved in various physiological processes such as neurotransmission, vasodilation, and immune response.
Dysregulation of NOS1 has been implicated in a range of diseases, including neurodegenerative disorders, cardiovascular diseases, and inflammatory conditions.By accurately measuring NOS1 levels, researchers can gain valuable insights into the molecular mechanisms underlying these diseases and potentially identify novel therapeutic targets. The Mouse NOS1 CLIA Kit from Assay Genie provides a reliable and efficient solution for studying NOS1 biology in preclinical research settings.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS1/nNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS1/nNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS1/nNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NOS1/nNOS. You can calculate the concentration of Mouse NOS1/nNOS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-99
96-111
98-116
Average (%)
91
104
106
1:4
Range (%)
103-117
93-108
87-100
Average (%)
109
99
94
1:8
Range (%)
100-113
103-118
97-111
Average (%)
107
109
104
1:16
Range (%)
104-116
99-114
98-112
Average (%)
110
105
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-111
102
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
87-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
109.21
291.9
978.5
107.17
312.45
998.02
Standard deviation
12.65
23.0
109.98
11.96
27.5
67.97
C V (%)
11.58
7.88
11.24
11.16
8.8
6.81
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NOS1/nNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NOS1/nNOS in samples. No significant cross-reactivity or interference between Mouse NOS1/nNOS and analogues was observed.
