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Mouse Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

SKU:
MODL00945
Product Type:
ELISA Kit
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
AWD,GAAD,NDPKA,NM23-H1,Nucleoside diphosphate kinase A,Granzyme A-activated DNase,Metastasis inhibition factor nm23,Tumor metastatic process-associated protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Mouse Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit

The Mouse Non-Metastatic Cells 1 Protein (NM23A) ELISA Kit is a powerful tool for accurately measuring the expression levels of NM23A in mouse cell samples. With high sensitivity and specificity, this kit ensures precise and consistent results, making it ideal for various research applications.NM23A, also known as NME1, is an important protein involved in regulating cell behavior and metastasis. By studying its expression levels, researchers can gain insights into cellular processes, including cell migration and invasion.

This makes the NM23A ELISA Kit a valuable asset for investigating cancer progression and potential therapeutic strategies.Overall, the Mouse NM23A ELISA Kit is a reliable and efficient solution for studying the role of NM23A in cell biology and disease pathogenesis. Its ease of use and accurate results make it a valuable tool for researchers in need of a reliable assay for detecting NM23A expression levels in mouse cell samples.

Product Name:Mouse Non Metastatic Cells 1, Protein NM23A Expressed In (NME1) ELISA Kit
Product Code:MODL00945
Size:96 Assays
Target:NME1
Synonyms:AWD, GAAD, NDPKA, NM23-H1, Nucleoside diphosphate kinase A, Granzyme A-activated DNase, Metastasis inhibition factor nm23, Tumor metastatic process-associated protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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