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Mouse Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

SKU:
MODL00950
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29477
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
NOS2A,INOS,HEP-NOS,I-NOS,Hepatocytes Oxide Synthase,Peptidyl-cysteine S-nitrosylase NOS2
Reactivity:
Mouse
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

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Mouse Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit

The Mouse Nitric Oxide Synthase 2 (inducible NOS2) ELISA Kit is specifically designed for the quantitative detection of inducible NOS2 levels in mouse serum, plasma, and cell culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.Inducible NOS2, also known as NOS2, is an enzyme involved in the production of nitric oxide, a signaling molecule that plays a critical role in immune response and inflammation.

Dysregulation of NOS2 has been linked to various diseases such as septic shock, cancer, and autoimmune disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Mouse Nitric Oxide Synthase 2 (inducible NOS2) ELISA Kit is a valuable tool for researchers looking to study the role of inducible NOS2 in health and disease, providing reliable and accurate data for their studies.

Product Name:Mouse Nitric Oxide Synthase 2, Inducible (NOS2) ELISA Kit
Product Code:MODL00950
Size:96 Assays
Target:NOS2
Synonyms:NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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