The Mouse NGB (Neuroglobin) CLIA Kit is a highly sensitive and specific assay designed for the accurate detection of neuroglobin levels in mouse serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Neuroglobin is a vital protein involved in neuroprotection, oxygen homeostasis, and neural cell survival.
It plays a significant role in conditions such as stroke, Alzheimer's disease, and traumatic brain injury, making it a valuable biomarker for studying these conditions and exploring potential treatments.Overall, the Mouse NGB (Neuroglobin) CLIA Kit is an essential tool for researchers seeking to investigate the role of neuroglobin in neurological disorders and develop novel therapeutic strategies.
Product Name:
Mouse NGB (Neuroglobin) CLIA Kit
SKU:
MOES00447
Target:
Mouse NGB (Neuroglobin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NGB. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NGB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NGB, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NGB. You can calculate the concentration of Mouse NGB in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
100-113
102-114
Average (%)
94
105
107
1:4
Range (%)
88-104
95-108
98-115
Average (%)
95
102
106
1:8
Range (%)
100-119
89-104
92-108
Average (%)
109
95
99
1:16
Range (%)
98-113
88-98
103-118
Average (%)
106
93
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
98
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
85-100
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.22
7.74
1.09
2.14
7.96
Standard deviation
0.1
0.18
0.82
0.11
0.18
0.64
C V (%)
9.62
8.11
10.59
10.09
8.41
8.04
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NGB concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NGB in samples. No significant cross-reactivity or interference between Mouse NGB and analogues was observed.
