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Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

SKU:
MODL00939
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P11672
Sensitivity:
0.285ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
LCN2,p25,Lipocalin 2,Oncogene 24p3,25 kDa alpha-2-microglobulin-related subunit of MMP-9,Siderocalin LCN2
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Urine, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit

The Mouse Neutrophil Gelatinase-Associated Lipocalin (NGAL) ELISA Kit is a powerful tool for the accurate measurement of NGAL levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.NGAL is a key protein involved in various physiological and pathological processes, such as kidney injury, inflammation, and infection. Its levels have been shown to be elevated in conditions like acute kidney injury, sepsis, and urinary tract infections, making it a valuable biomarker for studying these diseases and developing potential treatments.

With the Mouse NGAL ELISA Kit, researchers can confidently analyze NGAL levels in mouse samples, enabling a deeper understanding of disease mechanisms and potential therapeutic targets. This kit is a reliable and efficient solution for advancing research in the fields of nephrology, immunology, and inflammation.

Product Name:Mouse Neutrophil Gelatinase Associated Lipocalin (NGAL) ELISA Kit
Product Code:MODL00939
Size:96 Assays
Target:NGAL
Synonyms:LCN2, p25, Lipocalin 2, Oncogene 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, Siderocalin LCN2
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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