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Mouse Neutrophil Elastase ELISA Kit

SKU:
MOFI00993
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q3UP87
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
NE, Neutrophil Elastase, ELANE, PMN elastase, Medullasin, Bone marrow serine protease, Elastase-2
Reactivity:
Mouse
€649
Frequently bought together:

Description

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Mouse Neutrophil Elastase ELISA Kit

The Mouse Neutrophil Elastase ELISA Kit is specifically developed for the precise measurement of neutrophil elastase levels in mouse serum, plasma, and cell culture supernatants. Featuring high sensitivity and specificity, this kit ensures accurate and consistent results, making it an excellent tool for various research purposes.Neutrophil elastase is a key enzyme secreted by neutrophils, playing a crucial role in inflammatory responses and tissue damage. Dysregulation of neutrophil elastase activity has been linked to various diseases such as acute respiratory distress syndrome, cystic fibrosis, and chronic obstructive pulmonary disease.

Therefore, measuring neutrophil elastase levels can provide valuable insights into pathophysiological processes and potential therapeutic targets for these conditions.The Mouse Neutrophil Elastase ELISA Kit is a valuable resource for researchers studying inflammatory diseases, immune responses, and tissue remodeling processes in mouse models. Its reliable performance and easy-to-use protocol make it a valuable addition to any laboratory conducting experiments related to neutrophil biology and inflammation.

Product Name:Mouse Neutrophil Elastase ELISA Kit
Product Code:MOFI00993
Size:96 Assays
Alias:NE, Neutrophil Elastase, ELANE, PMN elastase, Medullasin, Bone marrow serine protease, Elastase-2
Detection Method:Sandwich ELISA
Application:This immunoassay kit allows for the in vitro quantitative determination of Mouse NE concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery: Matrices listed below were spiked with certain level of Mouse NE and the recovery rates were calculated by comparing the measured value to the expected amount of Mouse NE in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)90-10497
EDTA plasma(n=5)88-10595
UFH plasma(n=5)93-10397
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mouse NE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)88-102%86-102%85-100%
EDTA plasma(n=5)83-89%86-97%83-101%
UFH plasma(n=5)80-99%81-90%83-98%
Intra Assay:CV <8%
Inter Assay:CV <10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ3UP87
UniProt Protein Function:ELANE: Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. Defects in ELANE are a cause of cyclic haematopoiesis (CH); also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency. Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1). SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. Belongs to the peptidase S1 family. Elastase subfamily.
UniProt Protein Details:

Protein type:Protease; EC 3.4.21.37; Cell cycle regulation; Motility/polarity/chemotaxis; Cell surface

Cellular Component: cell surface; cytoplasm; extracellular space; secretory granule; transcriptional repressor complex

Molecular Function:cytokine binding; endopeptidase activity; heparin binding; hydrolase activity; peptidase activity; protease binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: acute inflammatory response to antigenic stimulus; defense response to bacterium; defense response to fungus; leukocyte migration; leukocyte migration during inflammatory response; negative regulation of chemokine biosynthetic process; negative regulation of interleukin-8 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; phagocytosis; positive regulation of immune response; positive regulation of interleukin-8 biosynthetic process; positive regulation of smooth muscle cell proliferation; proteolysis; response to lipopolysaccharide; response to UV; response to yeast

NCBI Summary:This gene encodes a member of the chymotrypsin-like family of serine protease enzymes that hydrolyzes a broad range of protein substrates including elastin. This gene is expressed by neutrophils where the encoded enzyme is stored in azurophil granules. Upon neutrophil activation, the active enzyme is released into the extracellular mileu. Mice lacking the encoded protein exhibit increased susceptibility to sepsis and death following intraperitoneal infection with Gram negative bacteria. This gene is located adjacent to a related proteinase gene on chromosome 10. [provided by RefSeq, Jul 2016]
UniProt Code:Q3UP87
NCBI GenInfo Identifier:114052444
NCBI Gene ID:50701
NCBI Accession:NP_056594.2
UniProt Related Accession:Q3UP87
Molecular Weight:
NCBI Full Name:neutrophil elastase
NCBI Synonym Full Names:elastase, neutrophil expressed
NCBI Official Symbol:Elane  
NCBI Official Synonym Symbols:NE; Ela2; F430011M15Rik  
NCBI Protein Information:neutrophil elastase
UniProt Protein Name:Neutrophil elastase
UniProt Synonym Protein Names:Elastase-2; Leukocyte elastase
UniProt Gene Name:Elane  
UniProt Entry Name:ELNE_MOUSE

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1. Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3. Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4. Add 0.1 ml of properly diluted sample (Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6. Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7. Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9. Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10. Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11. Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12. Add 90 µL of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13. Add 50 µL of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample Type Protocol
Serum:

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

Plasma:

Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.

Urine & Cerebrospinal Fluid:

Collect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.

Cell culture supernatant:

Collect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.

Cell lysates:

Solubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20°C.

Tissue homogenates:

The preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.

Tissue lysates:

Rinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.

Breast Milk:

Collect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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