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Mouse Neutrophil Activating Protein 3 (NAP3) ELISA Kit (MODL00920)

SKU:
MODL00920
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P12850
Sensitivity:
5.8pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Sandwich
Synonyms:
CXCL1,GRO1,GROa,GRO-A,MGSA-A,NAP3,SCYB1,Chemokine C-X-C-Motif Ligand 1,Melanoma Growth Stimulating Activity Alpha,Fibroblast Secretory Protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Neutrophil Activating Protein 3 (NAP3) ELISA Kit

The Mouse Neutrophil Activating Protein 3 (NAP3) ELISA Kit is specially designed for the precise detection of NAP3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.NAP3 is a key protein involved in activating neutrophils, important immune cells that play a crucial role in fighting infections and inflammation.

Understanding NAP3 levels can provide valuable insights into immune responses and inflammatory processes, making it a valuable tool for studying various diseases and potential therapeutic interventions.With its high quality and reliability, the Mouse NAP3 ELISA Kit is an indispensable resource for researchers interested in exploring the role of NAP3 in immune regulation and inflammatory diseases in mouse models.

Product Name:Mouse Neutrophil Activating Protein 3 (NAP3) ELISA Kit
Product Code:MODL00920
Size:96 Assays
Target:NAP3
Synonyms:CXCL1, GRO1, GROa, GRO-A, MGSA-A, NAP3, SCYB1, Chemokine C-X-C-Motif Ligand 1, Melanoma Growth Stimulating Activity Alpha, Fibroblast Secretory Protein
Detection Method:Sandwich
Range:15.625-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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