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Mouse Neuropeptide FF (NPFF) ELISA Kit

SKU:
MODL00953
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WVA8
Sensitivity:
0.048ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Competitive
Synonyms:
FMRFAL,Neuropeptide FF-Amide Peptide Precursor,Pro-FMRFamide-related neuropeptide FF
Reactivity:
Mouse
Sample Type:
Urine, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Mouse Neuropeptide FF (NPFF) ELISA Kit

The Mouse Neuropeptide FF (NPFF) ELISA Kit is a valuable tool for researchers studying neuropeptide FF levels in mouse serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit provides accurate and reproducible results for a variety of research applications.Neuropeptide FF is a neuropeptide involved in regulating pain perception, stress response, and cardiovascular functions. Dysregulation of neuropeptide FF has been linked to various neurological disorders, making it a significant biomarker for understanding and potentially treating these conditions.

Overall, the Mouse Neuropeptide FF (NPFF) ELISA Kit is a reliable and effective solution for researchers looking to investigate the role of neuropeptide FF in mouse models and advance their understanding of neurobiology and related disorders.

Product Name:Mouse Neuropeptide FF (NPFF) ELISA Kit
Product Code:MODL00953
Size:96 Assays
Target:NPFF
Synonyms:FMRFAL, Neuropeptide FF-Amide Peptide Precursor, Pro-FMRFamide-related neuropeptide FF
Detection Method:Competition
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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