The Mouse Neurogenin-3 (Neurog3) ELISA Kit offered by Assay Genie is specifically designed for the precise detection of Neurogenin-3 levels in mouse samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring reliable and consistent results for various research applications.Neurogenin-3, also known as Neurog3, is a key transcription factor that plays a crucial role in the development of the nervous system, specifically in the differentiation of pancreatic endocrine cells.
Dysregulation of Neurogenin-3 has been linked to various neurological disorders and diseases, making it a valuable biomarker for further research and potential therapeutic interventions.With its advanced technology and accurate results, the Mouse Neurogenin-3 (Neurog3) ELISA Kit is a valuable tool for researchers studying neurodevelopmental disorders, diabetes, and other related conditions. Get your kit today from Assay Genie and advance your research with confidence.
Product Name:
Mouse Neurogenin 3 (NEUROG3) ELISA Kit
Product Code:
MODL00933
Size:
96 Assays
Target:
NEUROG3
Synonyms:
Math4B, Atoh5, ngn3, bHLHa7, Class A basic helix-loop-helix protein 7, Protein atonal homolog 5
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-101
91
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
