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Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit

SKU:
MODL00928
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P08551
Sensitivity:
12.5pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CMT1F,CMT2E,NF-L,NF68,NFL,68 kDa neurofilament protein,Neurofilament triplet L protein
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Cerebrospinal Fluid And Other Biological Fluids
$779
Frequently bought together:

Description

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Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit

The Mouse Neurofilament Light Polypeptide (NEFL) ELISA Kit is specifically designed for the accurate measurement of NEFL levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for various research purposes.NEFL is a key protein found in neurons and is crucial for maintaining the structural integrity of nerve cells. Its levels can be indicative of neurodegenerative diseases such as Alzheimer's, Parkinson's, and multiple sclerosis.

By detecting NEFL levels, researchers can gain valuable insights into these conditions and potentially develop novel therapeutic strategies.Overall, the Mouse NEFL ELISA Kit is a valuable tool for studying neurological disorders and advancing our understanding of neurodegeneration. Its high performance and reliability make it a must-have for researchers in the field.

Product Name:Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit
Product Code:MODL00928
Size:96 Assays
Target:NEFL
Synonyms:CMT1F, CMT2E, NF-L, NF68, NFL, 68 kDa neurofilament protein, Neurofilament triplet L protein
Detection Method:Sandwich
Range:31.25-2,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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