null

Mouse Neuregulin 4 (NRG4) ELISA Kit (MODL00967)

(No reviews yet) Write a Review
SKU:
MODL00967
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9WTX4
Sensitivity:
0.128ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HRG4,Pro-NRG4,Pro-neuregulin-4, membrane-bound isoform
Reactivity:
Mouse
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649

Description

system_update_altDatasheet

Mouse Neuregulin 4 (NRG4) ELISA Kit

The Mouse Neuregulin-4 (NRG4) ELISA Kit is a reliable tool for precise measurement of NRG4 levels in mouse serum, plasma, and tissue culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring consistent and accurate results for a variety of research applications.NRG4 is a key regulator of various physiological processes, including metabolism and energy homeostasis. Dysregulation of NRG4 has been linked to metabolic disorders such as obesity and diabetes, highlighting its importance as a potential biomarker for studying these conditions and exploring therapeutic interventions.

Overall, the Mouse Neuregulin-4 (NRG4) ELISA Kit is an indispensable resource for scientists seeking to investigate the role of NRG4 in metabolic diseases and other related disorders.

Product Name:Mouse Neuregulin 4 (NRG4) ELISA Kit
Product Code:MODL00967
Size:96 Assays
Target:NRG4
Synonyms:HRG4, Pro-NRG4, Pro-neuregulin-4, membrane-bound isoform
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

0 Reviews

View AllClose

Related Products