The Mouse Neurofilament, Light Polypeptide (NEFL) ELISA Kit is innovatively developed to facilitate the precise quantification of NEFL levels in mouse biological samples.
NEFL, an essential component of the neuronal cytoskeleton, plays a critical role in neuronal function, axonal transport, and overall neuronal integrity. As a neurofilament protein, variations in NEFL levels can reflect neural damage, neurodegenerative processes, and neurological disorders, providing insights into neuronal health and pathology. This ELISA kit enables researchers to accurately measure NEFL levels, offering valuable information about neuronal injury, neurodegeneration, and neurodevelopment in mouse models. Quantitative assessment of NEFL can help unravel the underlying mechanisms of neurodegenerative diseases, monitor neural damage, and explore potential therapeutic interventions aimed at preserving neuronal function and health. With superior sensitivity and specificity, the Mouse NEFL ELISA Kit delivers reliable and reproducible results, empowering researchers to investigate neurofilament dynamics, neuronal disorders, and neuroprotective strategies with confidence. Manufactured under stringent quality control measures, this kit ensures robust performance and user-friendly operation, making it an indispensable tool for neuroscience research and preclinical investigations in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NEFL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NEFL. You can calculate the concentration of Mouse NEFL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
98-111
91-105
92-102
Average (%)
104
98
97
1:4
Range (%)
88-103
82-93
84-95
Average (%)
95
88
89
1:8
Range (%)
88-99
87-99
87-100
Average (%)
93
92
93
1:16
Range (%)
87-98
82-94
87-97
Average (%)
92
87
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-105
98
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
89-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.57
3.52
0.46
1.6
3.6
Standard deviation
0.02
0.08
0.18
0.03
0.08
0.12
C V (%)
4.35
5.1
5.11
6.52
5.0
3.33
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100 μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NEFL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NEFL in samples. No significant cross-reactivity or interference between Mouse NEFL and analogues was observed.
