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Mouse NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit

SKU:
AEES00220
Product Type:
ELISA Kit
ELISA Type:
Sandwich
Size:
96 Assays
Reactivity:
Mouse
Sensitivity:
0.94 ng/mL
Range:
1.56-100 ng/mL
Sample Type:
Serum, plasma and other biological fluids
$599
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Description

Mouse NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit

The Mouse NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit is a specialized assay designed to quantitatively measure the levels of Neutrophil Elastase, also known as Elastase-2, in mouse biological samples. Neutrophil Elastase is a crucial enzyme involved in various inflammatory processes, particularly within the immune system and neutrophil activation. This ELISA kit offers exceptional sensitivity and specificity, enabling accurate measurement of Neutrophil Elastase levels for researchers studying inflammatory responses, immune function, and related conditions.

With stringent quality control measures in place, the kit ensures robust performance, providing reliable and reproducible results. Its user-friendly design makes it an ideal choice for investigative studies and preclinical research in the field of inflammation and immune-related disorders.

Product Name:Mouse NE/ELA2 (Neutrophil Elastase/Elastase-2) ELISA Kit
Product Code:AEES00220
Assay Type:Sandwich
Format:96T
Assay Time:3.5h
Reactivity:Mouse
Detection Range:1.56-100 ng/mL
Sensitivity:0.94 ng/mL
Sample Type & Sample Volume:Serum, plasma and other biological fluids, 100μL
Specificity:This kit recognizes Mouse NE/ELA2 in samples. No significant cross-reactivity or interference between Mouse NE/ELA2 and analogues was observed.
Reproducibility:Both intra-CV and inter-CV are < 10%.
Application:This ELISA kit applies to the in vitro quantitative determination of Mouse NE/ELA2 concentrations in serum, plasma and other biological fluids.

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NE/ELA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NE/ELA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NE/ELA2. You can calculate the concentration of Mouse NE/ELA2 in the samples by comparing the OD of the samples to the standard curve.

Kit Components:

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)8 wells x 12 strips-20℃, 6 months
Reference Standard2 vials
Concentrated Biotinylated Detection Ab (100×)1 vial, 120 μL
Concentrated HRP Conjugate (100×)1 vial, 120 μL-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent1 vial, 20 mL2-8°C, 6 months
Biotinylated Detection Ab Diluent1 vial, 14 mL
HRP Conjugate Diluent1 vial, 14 mL
Concentrated Wash Buffer (25×)1 vial, 30 mL
Substrate Reagent1 vial, 10 mL2-8℃(Protect from light)
Stop Solution1 vial, 10 mL2-8℃
Plate Sealer5 pieces
Manual1 copy
Certificate of Analysis1 copy
1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
3. Aspirate and wash the plate for 3 times
4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
6. Add 50μL Stop Solution
7. Read the plate at 450nm immediately. Calculation of the results

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