The Mouse NCAD (Neural Cadherin) CLIA Kit is a cutting-edge assay designed for precise quantification of NCAD levels in mouse samples. The kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it perfect for a variety of research studies.NCAD, also known as Neural Cadherin, is a key protein involved in cell adhesion and neural development.
It plays a critical role in processes such as neuronal migration and synapse formation, making it a fundamental biomarker for studying neural development and neurological disorders. By measuring NCAD levels in mouse samples, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially discover new therapeutic targets.
Product Name:
Mouse NCAD (Neural Cadherin) CLIA Kit
SKU:
MOES00134
Target:
Mouse NCAD (Neural Cadherin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NCAD. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NCAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NCAD, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NCAD. You can calculate the concentration of Mouse NCAD in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
101-119
85-98
87-101
Average (%)
109
91
92
1:4
Range (%)
91-107
98-112
94-108
Average (%)
99
105
102
1:8
Range (%)
98-113
100-113
95-110
Average (%)
104
106
103
1:16
Range (%)
92-107
101-118
87-101
Average (%)
99
109
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-98
92
EDTA plasma (n=5)
85-100
92
Cell culture media (n=5)
85-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
90.94
230.33
717.23
98.59
237.27
717.56
Standard deviation
7.67
17.21
79.54
8.53
17.2
49.58
C V (%)
8.43
7.47
11.09
8.65
7.25
6.91
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NCAD concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NCAD in samples. No significant cross-reactivity or interference between Mouse NCAD and analogues was observed.
